1. SCI论文发表一览表

1.Yanfeng Liu, Hannes Link, Long Liu, Guocheng Du, Jian Chen & Uwe Sauer.A dynamic

pathway analysis approach reveals a limiting futile cycle inN-acetyl glucosamine

overproducingBacillus subtilis.

Nature Communications.2016, 7:11933. (IF=11.33)

2.Xiao-Wei Yu, Yan Xu, Rong Xiao.Lipases from the genus Rhizopus: Characteristics, expression, protein engineering and application.

Progress in Lipid Research.2016,64:57–68.(IF=11.24)

3.Minglong Shao, Xian Zhang,Zhiming Rao,Meijuan Xu,Taowei Yang,Hui Li,Zhenghong Xu and Shangtian Yang. Efficient testosterone production by engineeredPichia pastorisco-expressing human 17β-hydroxysteroid dehydrogenase type 3 andSaccharomyces cerevisiaeglucose 6-phosphate dehydrogenase with NADPH regeneration.

Green Chemristy.2016,18(6):1774-1784.(IF=8.506)

4.Peng Jin, Zhen Kang , Panhong Yuan, Guocheng Du, Jian Chen.Production of specific-molecular-weight hyaluronan by metabolically engineeredBacillus subtilis.

Metabolic Engineering.2016, 35:21-30.(IF=8.201)

5.Zaiwei Man, Zhiming Rao,Meijuan Xu, Jing Guo, Taowei Yang, Xian Zhang, Zhenghong Xu.Improvement of the intracellular environment for enhancing l-arginine production ofCorynebacterium glutamicumby inactivation of H2O2-forming flavin reductases and optimization of ATP supply.

Metabolic Engineering.2016,38:310-321.(IF=8.201)

6.Fubao Fuelbiol Sun,Xiaoqin Zhao, Jiapeng Hong, Yanjun Tang, Liang Wang, Haiyan Sun, Xiang Li, and Jinguang Hu.Industrially relevant hydrolyzability and fermentability of sugarcane bagasse improved effectively by glycerol organosolv pretreatment.

Biotechnology for Biofuels.2016, 9:59.(IF=6.444)

7.Bo-Bo Zhang Li Wang, Valérie Charles, Joanna C. Rooke, and Bao-Lian Su. Robust and biocompatible hybrid matrix with controllable permeability for microalgae encapsulation.

ACS Applied Materials & Interfaces.2016, 8(14): 8939–8946.(IF=7.145)

8.Hai-Ming Si,Fa Zhang,An-Ning Wu,Rui-Zhi Han,Guo-Chao Xu and Ye Ni. DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance inEscherichia coli.

Biotechnology Biofuels.2016, 9: 114.(IF=6.444)

9.Peng Jin, Zhen Kang,Junli Zhang, Linpei Zhang, Guocheng Du, and Jian Chen. Combinatorial evolution of enzymes and synthetic pathways using one-step PCR.

ACS Synthetic Biology.2016,5(3):259-268.(IF=6.076)

10.Peng Jin, Wenwen Ding, Guocheng Du, Jian Chen, and Zhen Kang. DATEL: A Scarless and Sequence-Independent DNA Assembly Method Using Thermostable Exonucleases and Ligase.

ACS Synthetic Biology.2016，5(9):1028-1032.(IF=6.076)

11.Jieyu Zhou,Guochao Xu,Ruizhi Han,Jinjun Dong,Weiguo Zhang,Rongzhen Zhang and Ye Ni. Carbonyl group-dependent high-throuhput screening and enzymatic characterization of diaromatic ketone reductase.

Catalysis Science & Technology.(2016) 6: 6320-6327. (IF=5.287)

12.Zhongyi Cheng,Wenjing Cui,Zhongmei Liu,Li Zhou,Min Wang,Michihiko Kobayashi and Zhemin Zhou. switch in a substrate tunnel for directing regioselectivity of nitrile hydratases towards alpha,omega-dinitriles.

Catalysis Science & Technology.2016,6(5):1292-1296. (IF=5.228)

13.Xinrui Zhao,Huijun Zou,Jian Chen,Guocheng Du and Jingwen Zhou. The modification of Gat1p in nitrogen catabolite repression to enhance non-preferred nitrogen utilization inSaccharomyces cerevisiae.

Scientific Reports.2016, 6:21603. (IF=5.228)

14.Yunfeng Zhang, Zhenmin Ling, Guocheng Du, Jian Chen & Zhen Kang. Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy.

Scientific Reports. 2016, 6:23158. (IF=5.228)

15.Zaiwei Man, Meijuan Xu, Zhiming Rao, Jing Guo, Taowei Yang, Xian Zhang & Zhenghong Xu. 7Systems pathway engineering ofCorynebacterium crenatumfor improved L-arginine production.

Scientific reports.2016,6:28629. (IF=5.228)

16.Zhen Fang, Juan Zhang, Guocheng Du & Jian Chen. Improved catalytic efficiency, thermophilicity, anti-salt and detergent tolerance of keratinase KerSMD by partially truncation of PPC domain thermophilicity, anti-salt and detergent tolerance of keratinase KerSMD by partially truncation of PPC domain.

Scientific Reports.2016, 6:27953. (IF=5.228)

17.Long Liu, Ningzi Guan, Gexin Zhu, Jianghua Li, Hyun-dong Shin, Guocheng Du & Jian Chen. Pathway engineering ofPropionibacterium jenseniifor improved production of propionic acid.

Scientific Reports.2016, 6:19963. (IF=5.228)

18.Peng Zhang, Guocheng Du, Huijun Zou, Guangfa Xie, Jian Chen, Zhongping Shi & Jingwen Zhou. Genome-wide mapping of nucleosome positions inSaccharomyces cerevisiaein response to different nitrogen conditions.

Scientific Reports.2016，6:33970. (IF=5.228)

19.Peng Zhang, Guocheng Du, Huijun Zou, Jian Chen, Guangfa Xie, Zhongping Shi & Jingwen Zhou. Effects of three permeases on arginine utilization inSaccharomyces cerevisiae.

Scientific Reports.2016, 6：20910. (IF=5.228)

20.Xinye Wang, Yao Nie, Xiaoqing Mu, Yan Xu & Rong Xiao. Disorder prediction-based construct optimization improves activity and catalytic efficiency ofBacillusnaganoensis pullulanase.

Scientific Reports.2016, 6: 24574. (IF=5.228)

21.Yuanyuan Xia, Wenjing Cui, Zhongmei Liu, Li Zhou, Youtian Cui, Michihiko Kobayashi & Zhemin Zhou. Construction of a subunit-fusion nitrile hydratase and discovery of an innovative metal ion transfer pattern.

SCIENTIFIC REPORTS.2016,6:19183. (IF=5.228)

22.Haixia Zhu, Guochao Xu, Kai Zhang, Xudong Kong, Ruizhi Han, Jiahai Zhou & Ye Ni. Crystal structure of tyrosine decarboxylase and identification of key residues involved in conformational swing and substrate binding.

Scientific Reports.2016, 6:27779. (IF=5.2228)

23.Pengcheng Chen, Shengtao Tao, Pu Zheng. Efficient and repeated production of succinic acid by turning sugarcane bagasse into sugar and support.

Bioresource Technology.2016，211: 406–413. (IF=4.917)

24.Ji-Cai Ding, Guo-Chao Xu, Rui-Zhi Han, Ye Ni. Biobutanol production from corn stover hydrolysate pretreated with recycled ionic liquid byClostridium saccharobutylicumDSM1386477.

Bioresource Technology.2016，199:228-234. (IF=4.917)

25.Shoushuai Feng,Hailin Yang and Wu Wang. Insights to the effects of free cells on community structure of attached cells and chalcopyrite bioleaching during different stages.

Bioresource Technology.2016，200:186-193. (IF=4.917)

26.Xinchao Yanga,Ke Wang, Huijun Wang, Jianhua Zhang, Zhonggui Mao. Ethanol fermentation characteristics of recycled water bySaccharomyces cerevisiaein an integrated ethanol-methane fermentation process.

Bioresource Technology.2016, 220,609-614. (IF=4.917)

27.Yang Song,Jianghua Li,Hyun-dong Shinc,Long Liu,Guocheng Du,Jian Chen. Biotechnological production of alpha-keto acids: current status and perspectives.

Bioresource Technology.2016. 219: 716-724. (IF=4.917)

28.Hongzhen Luo,Laibing Ge,Jingshu Zhang and Zhongping Shi. Enhancing acetone biosynthesis and acetone-butanol-ethanol fermentation performance by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiaeintegrated with exogenous acetate addition.

Bioresource Technology.2016, 200:111-120. (IF=4.917)

29.Jian Xu, Xian-Feng Su, Jia-Wei Bao, Hong-Jian Zhang, Xin Zeng, Lei Tang, Ke Wang, Jian-Hua Zhang, Xu-Sheng Chen, Zhong-Gui Mao. A novel cleaner production process of citric acid by recycling its treated wastewater.

Bioresource Technology.2016,211: 645-653. (IF=4.917)

30.Guo-Chao Xu, Ji-Cai Ding, Rui-Zhi Han, Jin-Jun Dong, Ye Ni. Enhancing cellulose accessibility of corn stover by deep eutectic solvent pretreatment for butanol fermentation.

Bioresource Technology.2016, 203: 364–369. (IF=4.917)

31.Chunshuai Wang, Chang Liu, Jibao Luo, Yaping Tian, Nandi Zhou. Direct electrochemical detection of kanamycin based on peroxidase-like activity of gold nanoparticles.

Analytica Chimica Acta.2016, 936: 75-82. (IF=4.712)

32.Ningzi Guan,Jianghua Li,Hyun-Dong Shin and Long Liu.Metabolic engineering of acid resistance elements to improve acid resistance and propionic acid production ofPropionibacterium jensenii.

Biotechnology and Bioengineering.2016. 113（6）: 1294-1304. (IF=4.243)

33.Xiao-Yu-Zhu Wang, Jin-Jun Dong, Guo-Chao Xu, Rui-Zhi Han, Ye Ni. Enhanced curdlan production with nitrogen feeding during polysaccharide synthesis byRhizobium radiobacter.

Carbohydrate Polymers .2016,150: 385-91. (IF=4.219)

34.Peng Jina, Linpei Zhanga, Panhong Yuan, Zhen Kanga, Guocheng Dua, Jian Chen. Efficient biosynthesis of polysaccharides chondroitin and heparosan by metabolically engineeredBacillus subtilis.

Carbohydrate Polymers.2016，140:424-432. (IF=4.219)

35.Yongmei Liu,Qiuya Gu,Fred Kwame Ofosu and Xiaobin Yu.Production,structural characterization and gel forming property of a new exopolysaccharide produced by Agrobacterium HX1126 using glycerol or D-mannitol as substrate.

Carbohydrate Polymers.2016,136:917-922. (IF=4.07)

36.Dianhui Wu, Ting Zhou, Xiaomin Li, Guolin Cai, Jian Lu. POD promoted oxidative gelation of water-extractable arabinoxylan through ferulic acid dimers. Evidence for its negative effect on malt filterability.

Food Chemistry.2016,197(A):422-426. (IF=4.052)

37.Zhen Fang;Juan Zhang;Baihong Liu;Guocheng Du;Jian Chen. Enhancement of the catalytic

efficiency and thermostability ofStenotrophomonas sp.keratinase KerSMD by domain

exchange with KerSMF.

Microbial Biotechnology.2016,9(1):35-46. (IF=3.991)

38.Yong-Dan Hu, Bo-Bo Zhang, Gan-Rong Xu,Xiang-Ru Liao,Peter C.K.Cheung. A mechanistic study on the biosynthetic regulation of bioactive metabolite Antroquinonol from edible and medicinal mushroomAntrodia camphorata.

Journal of Functional Foods.2016, 25: 70–79. (IF=3.973)

39.Qun Wu, Yu Kong and Yan Xu. Flavor profile of Chinese liquor is altered by interactions of intrinsic and extrinsic microbes.Applied and Environmental Microbiology.2016. 82(2): 422-430. (IF=3.823)

40.Xiaolong Hu,Hai Du,Cong Ren and Yan Xu. Illuminating Anaerobic Microbial Community and Co-occurrence Patterns across a Quality Gradient in Chinese Liquor Fermentation Pit Muds.

Applied and Environmental Microbiology.2016, 82, 2506-2515. (IF=3.823)

41.Wenjing Cui, Laichuang Han, Jintao Cheng, Zhongmei Liu, Li Zhou, Junling Guo and Zhemin Zhou. Engineering an inducible gene expression system forBacillus subtilisfrom a strong constitutive promoter and a theophyllineactivated synthetic riboswitch.

Microbial Cell Factories.2016, 15:199. (IF=3.744)

42.Meijuan Xu, Jingru Qin, Zhiming Rao, Hengyi You, Xian Zhang, Taowei Yang, Xiaoyuan Wang and Zhenghong Xu. Effect of Polyhydroxybutyrate (PHB) storage on L-arginine production in recombinantCorynebacterium crenatumusing coenzyme regulation.

Microbial Cell Factories.2016,15:15. (IF=3.744)

43.Rongzhen ZhangEmail author, Lei Wang, Yan Xu, Hongbo Liang, Xiaotian Zhou, Jiawei Jiang, Yaohui Li, Rong Xiao and Ye Ni. In situ expression of (R)carbonyl reductase rebalancing an asymmetric pathway improves stereoconversion efficiency of racemic mixture to (S)phenyl1,2ethanediol inCandida parapsilosisCCTCC M203011.

Microbial Cell Factories.2016, 15:143. (IF=3.744)

44.Meng Zhang, Xiao-Wei Yu, G. V. T. Swapna, Rong Xiao, Haiyan Zheng, Chong Sha, Yan Xu and Gaetano T. Montelione. Efficient production of 2H, 13C, 15N-enriched industrial enzymeRhizopus chinensislipase with native disulfide bonds.

Microbial Cell Factories.2016,15（1）:123. (IF=3.744)

45.Chengran Guan, Wenjing Cui, Jintao Cheng, Li Zhou, Zhongmei Liu and Zhemin Zhou. Development of an efficient autoinducible expression system by promoter engineering inBacillus subtilis.

Microbial Cell Factories .2016,15:66. (IF=3.744)

46.Fubao Fuelbiol Sun, Song Tang, Rukuan Liu, Yanjun Tang, Rui Wang, Zhanying Zhang, Zhongyuan Gao, Hui Li, Changzhu Li, Zhihong Xiao. Bio-refining fractionation of the Camellia oleifera Abel. hull with two-stage organosolv extraction.

Industrial Crops and Product.94 (2016) 790–799. (IF=3.449)

47.Yan Zhi, Qun Wu, Hai Du, Yan Xu. Biocontrol of geosmin-producingStreptomyces spp.by twoBacillusstrains from Chinese liquor.

International Journal of Food Microbiology.2016，231:1–9. (IF=3.445)

48.Hongwei Guo,Hui Wan,Hongwen Chen,Fang Fang,Song Liu and Jingwen Zhou. Proteomic analysis of the response of alpha-ketoglutarate-producer Yarrowia lipolytica WSH-Z06 to environmental pH stimuli.

Applied Microbiology and Biotechnology.2016, 100 (20): 8829-8841.

(IF=3.376)

49.Xianzhong Chen,Yan Xiao,Wei Shen,Algasan Govender,Liang Zhang,You Fan,Zhengxiang Wang. Displayof phytase on the cell surface ofSaccharomyces cerevisiaeto degrade phytate

phosphorus andimprove bioethanol production.

Applied Microbiology and Biotechnology.2016,100(5):2449-2458.

(IF=3.376)

50.Kang Zhen, Zhang Na, Zhang Yunfeng. Enhanced production of leech hyaluronidase by optimizing secretion and cultivation inPichia pastoris.

Applied Microbiology and Biotechnology.2016，100(2):707-717. (IF=3.376)

51.Guo Hongwei，Madzak Catherine，Du Guocheng，Zhou Jingwen. Mutagenesis of conserved active site residues of dihydrolipoamide succinyltransferase enhances the accumulation of alpha-ketoglutarate inYarrowia lipolytica.

Applied Microbiology and Biotechnology.2016, 100 (2): 649-659. (IF=3.376)

52.Lihua Zhang,Xianzhong Chen,Zhen Chen,Zezheng Wang,Shan Jiang,Li Li,Markus Pötter,Wei Shen,You Fan. Development of an efficient genetic manipulation strategy for sequential gene disruption and expression of different heterologous GFP genes in Candida tropicalis.

Applied Microbiology and Biotechnology.2016,100(22):9567-9580. (IF=3.376)

53.Shenghu Zhou,Peiran Liu,Jian Chen,Guocheng Du,Huazhong Li,Jingwen Zhou. Characterization of mutants of a tyrosine ammonia-lyase fromRhodotorula glutinis.

Applied Microbiology And Biotechnology.2016,100(24):10443-10452. (IF=3.376)

54.Chengran Guan, Wenjing Cui, Jintao Cheng, Rui Liu, Zhongmei Liu, Li Zhou, Zhemin Zhou. Construction of a highly active secretory expression system via an engineered dual promoter and a highly efficient signal peptide inBacillus subtilis.

N Biotechnol.2016,33(3):372-379. (IF=3.376)

55.Xinchao Yang,Ke Wang,Huijun Wang,Jianhua Zhang,Lei Tang and Zhonggui Mao. Control of pH by acetic acid and its effect on ethanol fermentation in an integrated ethanol–methane fermentation process.

RSC Advances.2016，6(63): 57902–57909. (IF=3.289)

56.Shoushuai Feng,Hailin Yang,Xiao Zhan and Wu Wang. Enhancement of dibenzothiophene biodesulfurization by weakening the feedback inhibition effects based on a systematic understanding of the biodesulfurization mechanism by Gordonia sp. through the potential “4S” pathway.

RSC Advances.2016，6（86）:82872-82881. (IF=3.289)

57.Jun Cheng, Guochao Xu,Ruizhi Han,Jinjun Donga and Ye Ni.Efficient access to L-phenylglycine using a newly identified amino acid dehydrogenase fromBacillus clausii.

RSC Advances.2016, 6(84): 80557 - 80563. (IF=3.289)

58.Yuhong Zhang,Yuanding You,Ziwei Xia,Xuyan Han,Yaping Tiana and Nandi Zhou. Graphene oxide-based selection and identification of ofloxacin-specific single-stranded DNA aptamers.

RSC Advances.2016, 6(101): 99540-99545. (IF=3.289)

59.Lei Chen,Wenna Cheng, Bo-Bo Zhang and P. C. K. Cheung. Structural and thermal analysis of a hyper-branched exopolysaccharide produced by submerged fermentation of mushroom mycelium.

RSC Advances.2016,6(113)：112260-112268. (IF=3.289)

60.Hailin Yang, Yitian Wang, Ling Zhang, Wei Shen. Heterologous expression and enzymatic characterization of fructosyltransferase fromAspergillus nigerinPichia pastoris.

New biotechnology.2016,33(1):164-70. (IF=3.199)

61.Mei Han, Chao Du, Zhi-Yuan Xu, He Qian, Wei-Guo Zhang. Rheological properties of phosphorylated exopolysaccharide produced bySporidiobolus pararoseusJD-2.

International Journal of Biological Macromolecules.

2016，88：603–613. (IF=3.138)

62.Chengtuo Niu,Linjiang Zhu,Xin Xu and Qi Li. Rational design of disulfide bonds increases thermostability of a mesophilic 1,3-1,4-β-glucanase fromBacillus tequilensis.

Plos One.2016, 11(4): e0154036. (IF=3.057)

63.Xunhang Li,Yanzhou Zhang,Zhiwen Wei,Zhengbing Guan,Yujie Cai and Xiangru Liao. Antifungal activity of isolatedBacillus amyloliquefaciensSYBC H47 for the biocontrol of peach gummosis.

PloS One.2016, 11(9): e0162125. (IF=3.05)

64.Yanzhou Zhang,Xunhang Li,Zhikui Hao,Ruchun Xi,Yujie Cai and Xiangru Liao. Hydrogen peroxide-resistant CotA and YjqC ofBacillus altitudinisspores are a promising biocatalyst for catalyzing reduction of sinapic acid and sinapine in rapeseed meal.

PloS One.2016, 11(6): e0158351. (IF=3.05)

65.Yingfang Ma, Wei Shen, Xianzhong Chen, Long Liu, Zhemin Zhou, Fei Xu and Haiquan Yang. Significantly enhancing recombinant alkaline amylase production inBacillus subtilisby integration of a novel mutagenesis-screening strategy with systems-level fermentation optimization.

Journal of Biological Engineering.2016, 10:13. (IF=3)

66.Xiaole Xia, Qingwen Zhang, Bin Zhang, Wuji Zhang and Wu Wang. Insights into the Biogenic Amine Metabolic Landscape during Industrial Semidry Chinese Rice Wine Fermentation.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY .2016, 64 (39)：7385–7393.

(IF=2.875)

67.Jianzhong Xu,Junlan Zhang,Mei Han,Weiguo Zhang. A method for simultaneous gene overexpression and inactivation in theCorynebacterium glutamicumgenome.

Journal of Industrial Microbiology & Biotechnology.

2016,43(10):1417–1427. (IF=2.745)

68.Youran Li,Liang Zhang,Zhongyang Ding,Zhenghua Gu,Guiyang Shi. Engineering of isoamylase: improvement of protein stability and catalytic efficiency through semi-rational design.

Journal of Industrial Microbiology & Biotechnology.

2016, 43(1): 3-12. (IF=2.745)

69.Minglong Shao,Xian Zhang,Zhiming Rao,Meijuan Xu,Taowei Yang,Hui Li,Zhenghong Xu,Shangtian Yang. A mutant form of 3-ketosteroid-Delta(1)-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095.

Journal of industrial microbiology and biotechnology.

2016,43(5):691-701. (IF=2.745)

70.Hua Li,Bao Shi Wang,You Ran Li,Liang Zhang,Zhong Yang Ding,Zheng Hua Gu,Gui Yang Shi. Metabolic engineering ofEscherichia coliW3110 for the production of l-methionine.

Journal of Industrial Microbiology & Biotechnology.

2016, 44(1): 75-88. (IF=2.745)

71.Junjie Yan, Weidong Liu, Yujie Li, Hui-Lin Lai, Yingying Zheng, Jian-Wen Huang, Chun-Chi Chen, Yun Chen, Jian Jin, Huazhong Lia, Rey-Ting Guo. Functional and structural analysis of Pichia pastoris-expressedAspergillus niger1,4-β-endoglucanase.

Biochem Biophys Res Commun .2016，475（1）：8-12. (IF=2.738)

72.Jinxia Wang,Zhenyu Zhang,Hedong Liu,Fubao Fuelbiol Sun,Chun Yue,Jinguang Hu,Chundi Wang. Construction and optimization of trans-4-hydroxy-L-proline production recombinant E. coli strain consuming the glycerol as carbon source.

Journal of Chemical Technology & Biotechnology.

2016, 91（9）: 2389–2398. (IF=2.738)

73.Li Zhou,Wen-jing Cui,Zhong-mei Liu,Zhe-min Zhou. Metabolic engineering strategies for D-lactate over production inEscherichia coli.

Journal of Chemical Technology & Biotechnology.

2016,91(3):576-584. (IF=2.738)

74.Yu Xin,Liushen Lu, Qing Wang, Ling Zhang, Yanjun Tong, Wu Wang. Coenzyme-like ligands for affinity isolation of cholesterol oxidase.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE

BIOMEDICAL AND LIFE SCIENCES .2016,1021:169-174. (IF=2.687)

75.Weizhu Zeng, Fang Fang, Song Liu, Guocheng Du, Jian Chen, Jingwen Zhou. Comparative genomics analysis of a series of Yarrowia lipolytica WSH-Z06 mutants with varied capacity for alpha-ketoglutarate production.

Journal of Biotechnology.2016, 239: 76-82. (IF=2.667)

76.Yanjun Tong,Shoushuai Feng, Yu Xin,Hailin Yang, Ling Zhang, Wu Wang,Wei Chen. Enhancement of soluble expression of codon-optimized Thermomicrobium roseum sarcosine oxidase inEscherichia colivia chaperone co-expression.

Journal of Biotechnology.2016,218:75-84. (IF=2.667)

77.Junjun Wu,Xia Zhang,Mingsheng Dong,Jingwen Zhou. Stepwise modular pathway engineering ofEscherichia colifor efficient one-step production of (2S)-pinocembrin.

Journal of Biotechnology.2016, 231: 183-192. (IF=2.667)

78.Sen Yang,Zhen Kang,Wenlong Cao,Guocheng Du,Jian Chen. Construction of a novel, stable, food-grade expression system by engineering the endogenous toxin-antitoxin system inBacillus subtilis.

Journal of Biotechnology.2016，219:40-47. (IF=2.667)

79.Guo-Chao Xu, Ling-Ling Zhang, Ye Ni. Enzymatic preparation of D-phenyllactic acid at high space-time yield with a novel phenylpyruvate reductase identified fromLactobacillus spCGMCC 9967.

Journal of Biotechnology .2016, 222: 29–37. (IF=2.667)

80.Ye Liu,Xu Gong,Cui Wang,Guocheng Du,Jian Chen,Zhen Kang. Production of glucaric acid from myo-inositol in engineeredPichia pastoris.

Enzyme and Microbial Technology.2016，91:8-16. (IF=2.624)

81.Shuiqing Long, Xian Zhang, Zhiming Rao, Kaiyue Chen, Meijuan Xu, Taowei Yang, Shangtian Yang. Amino acid residues adjacent to the catalytic cavity of tetramer L-asparaginase II contribute significantly to its catalytic efficiency and thermostability.

Enzyme And Microbial Technology.2016，82:15-22. (IF=2.624)

82.Fubao Fuelbiol Sun,Renhui Bai,Huimin Yang,Fei Wang,Jing He,Chundi Wang,Maobing Tu. Heterologous expression of codon optimized Trichoderma reesei Cel6A inPichia pastoris.

Enzyme and Microbial Technology.2016，92：107–116. (IF=2.624)

83.Yaohui Li, Rongzhen Zhang, Yan Xu, Rong Xiao, Lei Wang, Xiaotian Zhou, Hongbo Liang, Jiawei Jiang. Efficient bioreduction of 2-hydroxyacetophenone to (S)-1-phenyl-1,2-ethanediol through homologous expression of (S)-carbonylreductase II in Candida parapsilosis CCTCC M203011.

Process Biochemistry.2016, 51(9): 1175–1182. (IF=2.529)

84.Xu-Sheng Chen, Yang Gao, Bin Zhen, Dai Han, Jian-Hua Zhang, Zhong-Gui Mao. Separation and purification of ε-poly-l-lysine from fermentation broth.

Process Biochemistry.2016,51:134–141. (IF=2.529)

85.Yupeng Han,Jinjing Wang,Yongxian Li,Xueliang Wang,Qi Li. Heterologous expression of Hordeum vulgare protein Z4 inPichiapastorisshows increased structural stability.

Process biochemistry.2016, 51(7): 828-837. (IF=2.529)

86.Hua Li, Baoshi Wang, Linghuan Zhu, Shi Cheng, Youran Li, Liang Zhang, Zhong Yang Ding, Zheng Hua Gu, Gui Yang Shi. Metabolic engineering ofEscherichia coliW3110 for L-homoserine production.

Process Biochemistry.2016, 51(12): 1973-1983. (IF=2.529)

87.Huaxiang Deng, Ruijie Gao, Jiajun Chen, Xiangru Liao, , Yujie Cai. An efficient polyethylene glycol-mediated transformation system of lentiviral vector inShiraia bambusicola.

Process Biochemistry.2016, 51(10):1357-1362. (IF=2.516)

88.Huaxiang Deng, Jiajun Chen, Ruijie Gao, Xiangru Liao and Yujie Cai. Adaptive Responses to Oxidative Stress in theFilamentous Fungal Shiraia Bambusicola.

Molecules.2016, 21(9)：1118. (IF=2.465)

89.Panpan Wang,Yu Xia,Jianghua Li,Zhen Kang,Jingwen Zhoua,Jian Chen. Overexpression of pyrroloquinoline quinone biosynthetic genes affects L-sorbose production in Gluconobacter oxydans WSH-003.

Biochemical Engineering Journal.2016, 112: 70-77. (IF=2.463)

90.Jianzhong Xu,Mei Han,Xidong Ren,Weiguo Zhang. Modification of aspartokinase III and dihydrodipicolinate synthetase increases the production of l-lysine inEscherichia coli.

Biochemical Engineering Journal.2016，114：79–86. (IF=2.463)

91.Song Liu,Miao Wang,Guocheng Du and Jian Chen. Improving the active expression of transglutaminase inStreptomyces lividansby promoter engineering and codon optimization.

BMC Biotechnology.2016,16(1):75. (IF=2.452)

92.Bin Li, Yao Nie, Xiaoqing Mu, Yan Xu.Denovo construction of multi-enzyme system for one-potderacemization of (R,S)-1-phenyl-1,2-ethanediol by stereoinversionof (S)-enantiomer to the corresponding counterpart.

Journal of Molecular Catalysis B: Enzymatic.2016, 129: 21-28. (IF=2.189)

93.Guo-Chao Xu, Ming-Hui Tang, Ye Ni. Asymmetric synthesis of lipitor chiral intermediate using a robust carbonyl reductase at high substrate to catalyst ratio.

Journal of Molecular Catalysis B:Enzymatic. 2016, 123：67–72. (IF=2.189)

94.Ting Jiang,Chao Kang,Xiao-Wei Yu,Yan Xu. High-level expression of prolyl endopeptidase inPichia pastorisusing PLA(2)as a fusion partner.

Journal of Molecular Catalysis B: Enzymatic.2016,125:81-87. (IF=2.189)

95.Yu Deng, Mingxue Sun, Sha Xu, Jingwen Zhou. Enhanced (S)-linalool production by fusion expression of farnesyl diphosphate synthase and linalool synthase inSaccharomyces cerevisiae.

Journal of Applied Microbiology.2016, 121 (1): 187-195. (IF=2.156)

96.Huaxiang Deng,Ruijie Gao,Xiangru Liao,Yujie Cai. Reference genes selection and relative expression analysis fromShiraia sp.SUPER-H168 productive of hypocrellin.

Gene.2016, 580(1):67-72. (IF=2.138)

97.Xin Zeng,Xu-Sheng Chen,Xi-Dong Ren,Liang Wang,Yang Gao,Zhong-Gui Mao. Improved ε-poly-L-lysine productivity partly resulting from rapid cell growth in cultures using a glucose–glycerol mixed carbon source.

Engeering in Life Science.2016, 16, 443–452. (IF=2.119)

98.Jingke Xue,Jing Liu,Chunshuai Wang,Yaping Tian and Nandi Zhou. Simultaneous electrochemical detection of multiple antibiotic residues in milk based on aptamers and quantum dots.

Analytical Methods.2016, 8(9): 1981-1988. (IF=1.915)

99.Jian Xu,Yang-Qiu Chen,Hong-Jian Zhang,Lei Tang,Ke Wang,Jian-Hua Zhang,Xu-Sheng Chen,Zhong-Gui Mao. Effect of propionic acid on citric acid fermentation in an integrated citric acid-methane fermentation process.

Bioprocess Biosystems Engineering.2016，39(3)：391-400. (IF=1.901)

100.Mei Han,Zhi-yuan Xu,Chao Du,He Qian,Wei-Guo Zhang. Effects of nitrogen on the lipid and carotenoid accumulation of oleaginous yeastSporidiobolus pararoseus.

Bioprocess And Biosystem Engineering.2016, 39(9):1425–1433. (IF=1.901)

101.Zheng-Bing Guan,Hai-Xi Yi,Hong Zhao,Yan Shui,Yu-Jie Cai,Xiang-Ru Liao. Cell division cycle 2 participates in eyestalk ablation-induced ovarian maturation ofProcambarus clarkii.

Aquaculture.2016,468:115-119. (IF=1.893)

102.Fang Fang, Tingting Feng, Guocheng Du & Jian Chen. Evaluation of the impact on food safety of a Lactobacillus coryniformis strain from pickled vegetables with degradation activity against nitrite and other undesirable compounds.

Food Additives And Contaminants Part A-Chemistry Anal Ysis

Control Exposure & Risk Assessment .2016,33(4):623-630. (IF=1.878)

103.Xinyao lu,Yuqin Wang,Hong Zong,Hao Ji,Bin Zhuge,Zhuoli Dong. Bioconversion of L-phenylalanine to 2-phenylethanol by the novel stress-tolerant yeastCandida glycerinogenesWL2002-5.

Bioengineered.2016,7(6):418-423. (IF=1.87)

104.Xinyao Lu, Guangjian Ji, Hong Zong & Bin Zhuge. The role of budABC on 1,3-propanediol and 2,3-butanediol production from glycerol inKlebsiella pneumoniaeCICIM B0057.

Bioengineered.2016,7(6):439-444. (IF=1.87)

105.Lin Peng, Xiao Yu, Chengyuan Li, Yanfei Cai, Yun Chen, Yang He. Enhanced recombinant factor VII expression in Chinese hamster ovary cells by optimizing signal peptides and fed-batch medium.

Bioengineered.2016,7（3）：189–197. (IF=1.87)

106.Dianhui, Wu；Xiaomin, Li；Jian, Lu；Jian, Chen；Liang, Zhang；Guangfa, Xie. Constitutive expression of the DUR1,2 gene in an industrial yeast strain to minimize ethyl carbamate production during Chinese rice wine fermentation.

FEMS Microbiology Letters. 2016,363:fnv214. (IF=1.858)

107.Jin-Jun Dong,Ji-Cai Ding,Yun Zhang,Li Ma,Guo-Chao Xu,Rui-Zhi Han,Ye Ni. Simultaneous saccharification and fermentation of dilute alkaline-pretreated corn stover for enhanced butanol production byClostridium saccharobutylicumDSM 13864.

FEMS Microbiology Letters. 2016，363（4）. (IF=1.858)

108.Lei Chen,Changjiang Yu,Yubin Ma,Hua Xu,Shumin Wang,Yu Wang,Xingxun Liud,Gongke Zhou. Insights into the structural and physicochemical properties of small granular starches from two hydrophyte duckweeds, Spirodela oligorrhiza and Lemna minor.

Carbohydrate Research.2016,435：208-214. (IF=1.817)

109.Xinyao lu,Guangsheng Wang,Yue Feng,Song Liu,Xiaoman Zhou,Guocheng Du,Jian Chen. The N-terminal α-helix domain of Pseudomonas aeruginosa lipoxygenase is required for its soluble expression inEscherichia colibut not essential for catalysis.

Journal of microbiology and biotechnology.2016,26(10):1701–1707.

(IF=1.685)

110.Xinyao Lu , Xiaomeng Fu , Hong Zong , Bin Zhuge. Overexpressions of xylA and xylB in Klebsiella pneumoniae Lead to Enhanced 1,3-Propanediol Production by Cofermentation of Glycerol and Xylose.

Journal of microbiology and biotechnology.2016, 26(7), 1252–1258.

(IF=1.685)

111.Linpei Zhang,Hao Huang,Hao Wang,Jian Chen,Guocheng Du,Zhen Kang. Rapid evolution of hyaluronan synthase to improve hyaluronan production and molecular mass inBacillus subtilis.

Biotechnology Letters.2016,38(12):2103-2108. (IF=1.639)

112.Le Kang,Yajun Bai,Yujie Cai,Xiaohui Zheng. Discovery of Novel Feruloyl Esterase Activity of Bioh inEscherichia ColiBl21(De3).

Biotechnology Letters.2016,38(6): 1009-1013. (IF=1.639)

113.Jiran Zhang,Guocheng Du,Jian Chen,Fang Fang. Characterization of aBacillus amyloliquefaciensstrain for reduction of citrulline accumulation during soy sauce fermentation.

Biotechnology Letters.2016,38(10):1723-31. (IF=1.639)

114.Jie Bi,Song Liu,Guocheng Du,Jian Chen. Bile salt tolerance ofLactococcus lactisisenhanced by expression of bile salt hydrolase thereby producing less bile acid in the cells.

Biotechnology Letters.2016,38(4):659-65. (IF=1.639)

115.Liang Wang,Xusheng Chen,Guangyao Wu,Xin Zeng,Xidong Ren,Shu Li,Lei Tang,Zhonggui Mao. Genome Shuffling and Gentamicin-Resistance to Improve ε-Poly-l-Lysine Productivity ofStreptomyces albulusW-156Applied Biochemistry & biotechnologyApplied.

Biochemistry & biotechnology.(IF=1.606)

116.Guo-Chao Xu,Lei Li,Rui-Zhi Han,Jin-Jun Dong,Ye Ni. Characterization and Soluble Expression of D-Hydantoinase fromPseudomonas fluorescensfor the Synthesis of D-Amino Acids.

Applied Biochemistry and Biotechnology.2016,179(1): 1–15. (IF=1.606)

117.Guo-Chao Xu,Ya-Qian Bian,Rui-Zhi Han,Jin-Jun Dong,Ye Ni. Cloning, Expression, and Characterization of budC Gene Encoding meso-2,3-Butanediol Dehydrogenase fromBacillus licheniformis.

Applied Biochemistry and Biotechnology.2016, 178(3): 604–617. (IF=1.606)

118.Junli Zhang,Zhen Kang,Wenwen Ding,Jian Chen,Guocheng Du. Integrated Optimization of the In Vivo Heme Biosynthesis Pathway and the In Vitro Iron Concentration for 5-Aminolevulinate Production.

Applied Biochemistry and Biotechnology.2016,178(6):1252-1262. (IF=1.606)

119.Li Zhou,Can Deng,Wen-Jing Cui,Zhong-Mei Liu,Zhe-Min Zhou. Efficient L-Alanine Production by a Thermo-Regulated Switch inEscherichia coli.

Applied Biochemistry and Biotechnology.2016,178(2):324-337. (IF=1.606)

120.Qian Zhang,Xiaohong Zha,Nandi Zhou,Yaping Tian. Preparation of crosslinked enzyme aggregates (CLEAs) of acid urease with urethanase activity and their application.

Journal of Basic Microbiology.2016,56（4）：422-431. (IF=1.585)

121.Xinyao Lu,Shuying He,Hong Zong,Jian Song,Wen Chen,Bin Zhuge.Improved 1, 2, 4-butanetriol production from an engineeredEscherichia coliby co-expression of different chaperone proteins.

World Journal of Microbiology and Biotechnology.2016, 32(9): 149.

(IF=1.532)

122.Hongyu Yang,Qiang Zhu,Nandi Zhou,Yaping Tian. Optimized expression of prolyl aminopeptidase inPichia pastorisand its characteristics after glycosylation.

World Journal of Microbiology and Biotechnology.2016,32（11）：176.

(IF=1.532)

123.Hao Ji,Bin Zhuge,Hong Zong,Xinyao Lu,Huiying Fang,Jian Zhuge. Role of CgHOG1 in Stress Responses and Glycerol Overproduction of Candida glycerinogenes.

Current microbiology.2016, 73(6): 827-833. (IF=1.519)

124.Mengxu Zhu,Wenlai Fan,Yan Xu,Qingyun Zhou. 1,1‐Diethoxymethane and methanethiol as age markers in Chinese roasted‐sesame‐like aroma and flavour type liquor.

European Food Research and Technology.2016, 242(11):1985-1992.

(IF=1.433)

125.Yong- Dan Hu,Rui-Qiu Lu,Xiang-Ru Liao,Gan-Rong Xu. Stimulating the biosynthesis of antroquinonol by addition of effectors and soybean oil in submerged fermentation ofAntrodia camphorata.

Biotechnology and Applied Biochemistry.2016, 63(3): 398–406. (IF=1.429)

126.Hong Zong,Cheng Zhang, Bin Zhuge,Xinyao lu,Huiying Fang,Jin Sun. Effects of xylitol dehydrogenase (XYL2) on xylose fermentation by engineeredCandida glycerinogenes.

Biotechnol Appl Biochem.2016,DOI: 10.1002/bab.1514. (IF=1.429)

127.Wan Song,Yao Nie,Xiao Qing Mu,Yan Xu. Enhancement of extracellular expression of Bacillus naganoensis pullulanase from recombinantBacillus subtilis: Effects of promoter and host.

Protein Expression and Purification.2016, 124: 23-31. (IF=1.407)

128.Jintao Cheng,Chengran Guan,Wenjing Cui,Li Zhou,Zhongmei Liu,Weijiang Li,Zhemin Zhou. Enhancement of a high efficient autoinducible expression system inBacillus subtilisby promoter engineering.

Protein Expression and Purification.2016,127:81-87. (IF=1.407)

129.Mayur Joshi, Harshad Patel, Shilpa Gupte, Akshaya Gupte, Nutrient Optimization Using Response Surface Methodology for Simultaneous Biomass and Bioactive Compound Production by Lion's Mane Medicinal Mushroom, Hericium erinaceus (Agaricomycetes).

International Journal Of Medicinal Mushrooms.2016;18(3):215-26.

(IF=1.357)

130.Jianzhong Xu,Junlan Zhang,Dongdong Liu,Weiguo Zhang. Increased glucose utilization and cell growth ofCorynebacterium glutamicumby modifying the glucosespecific phosphotransferase system (PTSGlc) genes.

Canadian Journal of Microbiology.2016，62（12）：983-992. (IF=1.335)

131.Jian-zhong Xu,Wei-guo Zhang. Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression.

Journal of Zhejiang University-SCIENCE B (Biomedicine &

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2016 17(2):83-99. (IF=1.303)

132.Yanzhou Zhang,Xunhang Li,Ruchun Xi,Zhengbing Guan,Yujie Cai,Xiangru Liao. Characterization of an acid-stable catalase KatB isolated fromBacillus altitudinisSYBC hb4

Annals of Microbiology2016,66(1):131-141. (IF=1.232)

133.Yanzhou Zhang,Haiye Luan,Zhiwen Wei,Zhikui Hao,Ruchun Xi,Xiangru Liao. Exploiting of honey-associatedBacillusstrains as plant-growth promoting bacteria for enhancing barley growth in rare earth tailings.

Annals of microbiology.2016, 66(2):559-568. (IF=1.232)

134.Cheng Zhang,Hong Zong,Bin Zhuge,Xinyao Lu,Huiying Fang,Jiali Zhu,Jian Zhuge. Protoplast preparation and polyethylene glycol (PEG)-mediated transformation ofCandida glycerinogenes.

Biotechnology and Bioprocess Engineering.2016, 21(1): 95-102.

(IF=1.211)

135.Jun Li,Hong Zong,Bin Zhuge,Xinyao Lu,Huiying Fang,Jin Sun. Immobilization ofAcetobacter sp.CGMCC 8142 for Efficient Biocatalysis of 1, 3-propanediol to 3-hydroxypropionic Acid.

Biotechnology and Bioprocess Engineering.2016,21(4):523-530. (IF=1.211)

136.Ming Li, Yao Nie, Xiao Qing Mu, Rongzhen Zhang & Yan Xu. Highly selective anti-Prelog synthesis of optically active aryl alcohols by recombinantEscherichia coliexpressing stereospecific alcohol dehydrogenasePreparative.

Biochemistry and Biotechnology.2016, 46(5): 429-433. (IF=1.114)

137.Gong Cheng, Jianzhong Xu, Xiuhua Xia, Yanfeng Guo, Kai Xu, Cunsheng Su and Weiguo Zhang. Breeding L-arginine-producing strains by a novel mutagenesis method: Atmospheric and room temperature plasma (ARTP).

Preparative Biochemistry and Biotechnology.2016, 46（5）: 509–516.

(IF=1.114)

138.Dianhui Wu, Xiaomin Li, Jian Lu, Jian Chen, Guangfa Xie, Liang Zhang.The overexpression of DUR1,2 and deletion of CAR1 in an industrialSaccharomyces cerevisiaestrain and effects on nitrogen catabolite repression in Chinese rice wine production.

Journal of the Institute of Brewing.2016,122(3):480-485. (IF=1.017)

139.Guolin Cai,Lihua You, Xiaomin Li,Dianhui Wu,Jian Lu. Cultivar discrimination/segregation of representative Australian malting barley by quantitative real-time PCR using seed hordein marker.

Journal of the Institute of Brewing. 2016,122(4):700-705. (IF=1.017)

140.Guolin Cai,Xiaomin Li,Chendong Zhang,Ming Zhang,Jian Lu. Dextrin as the main turbidity components in wort produced from major malting barley cultivars of Jiangsu province in China.

Journal of the Institute of Brewing.2106,122(3):543-546. (IF=1.017)

141.Shuxia Huang,Junhong Yu, Hua Yin,Jian Lu,Jianjun Dong,Xiaomin Li,Shumin Hu,Jia Liu. Optimization of kilning progress for equilibrating multiple parameters that strictly affect malt flavour and sensory evaluation.

Journal of the Institute of Brewing.2016,122(4): 706-713. (IF=1.017)

142.Xian Zhang,Zhiming Rao,Lele Zhang,Meijuan Xu,Taowei Yang. Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineeredBacillus subtilisco-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration.

SpringerPlus.2016,5:1207. (IF=0.982)

143.Yu-Jie Cai,Zheng-Bing Guan,Yan Shui,Kang Chen,Xiang-Ru Liao,Jun Yin. Hepatopancreas and ovary are the sites of vitellogenin synthesis in female red swamp crayfish (Procambarus clarkii).

Journal of Crustacean Biology.2016,36(5):637-641. (IF=0.922)

144.Ke Wang, Jianhua Zhang, Zhonggui Mao.Reusing the One-Stage Thermophilic Anaerobic Digestion Effluent for Cassava Ethanol Production.

Journal of Biobased Materials and Bioenergy.2016,10(4): 317–321.

(IF=0.635)

145.Yupeng Han, Jinjing Wang, Yongxian Li, Hao Li, Qi Li.Purification and Structural Characterization of Protein Z4 from Malt.

Journal Of The American Society Of Brewing Chemists.

2016, 74(2): 145-153. (IF=0.492)