Scientific research activities |
Selected Projects [1] National Natural Science Foundation of China (No. 32171261 and 21406088) [2] National Key Research and Development Program of China (No. 2021YFC2100900) [3] Natural Science Foundation of Jiangsu Province (No. BK20140133) [4] National High Technology Research and Development Program of China (No. 2014AA021501) [5] Fundamental Research Funds for the Central Universities (No. JUSRP11563)
Selected Recent Publications [1] Yao Z-Y,Gong J-S*, Liu Y-R, Jiang J-Y, Zhang Y-S, Su C, Li H, Kang C-L, Liu L, Xu Z-H, Shi J-S*. Genetic variation reveals the enhanced microbial hyaluronan biosynthesis via atmospheric and room temperature plasma [J]. Carbohydrate Polymers 2023, 312:120809. [2] Xiang Z-X,Gong J-S*, Li H, Shi W-T, Jiang M, Xu Z-H, Shi J-S*. Heterologous expression, fermentation strategies and molecular modification of collagen for versatile applications[J]. Critical Reviews in Food Science and Nutrition 2023, 63: 5268–5289. [3] Chen J-P,Gong J-S*, Su C, Li H, Xu Z-H, Shi J-S. Improving the soluble expression of difficult-to-express proteins in prokaryotic expression system via protein engineering and synthetic biology strategies [J]. Metabolic Engineering 2023, 78:99-114. [4] Liu Y,Gong J-S*, Marshall G, Su C, Hall M, Li H, Xu G-Q, Shi J-S, Xu Z-H. Protein engineering of NADH pyrophosphatase for efficient biocatalytic production of reduced nicotinamide mononucleotide [J]. Frontiers in Bioengineering and Biotechnology 2023, 11:1159965. [5] Zhang P,Gong J-S*, Xie Z-H, Su C, Zhang X-M, Rao Z-M, Xu Z-H, Shi J-S*. Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin [J]. Synthetic and Systems Biotechnology 2023, 8:273-280. [6] Wang Z-K,Gong J-S*, Feng D-T, Su C, Li H, Rao Z-M, Lu Z-M*, Shi J-S, Xu Z-H. Geometric Remodeling of Nitrilase Active Pocket Based on ALF-Scanning Strategy To Enhance Aromatic Nitrile Substrate Preference and Catalytic Efficiency [J]. Applied and Environmental Microbiology 2023, 89:e0022023. [7] Zhang Y-S,Gong J-S*, Yao Z-Y, Jiang J-Y, Su C, Li H, Kang C-L, Liu L, Xu Z-H, Shi J-S. Insights into the source, mechanism and biotechnological applications of hyaluronidases [J]. Biotechnology Advances 2022, 60:108018. [8] Xiang Z-X,Gong J-S*, Shi J-H, Liu C-F, Li H, Su C, Jiang M, Xu Z-H, Shi J-S*. High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris [J]. Bioresources and Bioprocessing 2022, 9:117. [9] Su C,Gong J-S*, Qin A, Li H, Li H, Qin J, Qian J-Y, Xu Z-H, Shi J-S*. A combination of bioinformatics analysis and rational design strategies to enhance keratinase thermostability for efficient biodegradation of feathers [J]. Science of the Total Environment 2022, 818:151824. [10] Zhang P,Gong J-S*, Qin J, Li H, Hou H-J, Zhang X-M, Xu Z-H, Shi J-S*. Phospholipids (PLs) know-how: exploring and exploiting phospholipase D for its industrial dissemination [J]. Critical Reviews in Biotechnology 2021, 41:1257-1278. [11] Yao Z-Y, Qin J,Gong J-S*, Ye Y-H, Qian J-Y, Li H, Xu Z-H, Shi J-S*. Versatile strategies for bioproduction of hyaluronic acid driven by synthetic biology [J]. Carbohydrate Polymers 2021, 264:118015. [12] Wang Z-K,Gong J-S*, Qin J, Li H, Lu Z-M, Shi J-S, Xu Z-H. Improving the Intensity of Integrated Expression for Microbial Production [J]. ACS Synthetic Biology 2021, 10:2796-2807. [13] Su C,Gong J-S*, Qin J, Li H, Li H, Xu Z-H, Shi J-S*. The tale of a versatile enzyme: Molecular insights into keratinase for its industrial dissemination[J]. Biotechnology Advances 2020, 45:107655. [14] Su C,Gong J-S*, Qin J, He J-M, Zhou Z-C, Jiang M, Xu Z-H, Shi J-S*. Glutathione enables full utilization of wool wastes for keratin production and wastewater decolorization[J]. Journal of Cleaner Production 2020, 270:122092. [15]Gong J-S, Ye J-P, Tao L-Y, Su C, Qin J, Zhang Y-Y, Li H, Li H, Xu Z-H, Shi J-S. Efficient keratinase expression via promoter engineering strategies for degradation of feather wastes[J]. Enzyme and Microbial Technology 2020, 137:109550. [16] Su C,Gong J-S*, Sun Y-X, Qin J, Zhai S, Li H, Li H, Lu Z-M, Xu Z-H, Shi J-S*. Combining Pro-peptide Engineering and Multisite Saturation Mutagenesis To Improve the Catalytic Potential of Keratinase [J]. ACS Synthetic Biology, 2019, 8 (2):425-433. [17]Gong J-S, Zhang Q, Gu B-C, Dong T-T, Li H, Li H, Lu Z-M, Shi J-S*, Xu Z-H*. Efficient biocatalytic synthesis of nicotinic acid by recombinant nitrilase via high density culture [J]. Bioresource Technology, 2018, 260:427-431. [18] Zhou W-B,Gong J-S*, Hou H-J, Li H, Lu Z-M, Xu H-Y*, Xu Z-H, Shi J-S. Mining of a phospholipase D and its application in enzymatic preparation of phosphatidylserine [J]. Bioengineered, 2018, 9 (1):80-89. [19]Gong, J.-S.; Shi, J.-S.; Lu, Z.-M.; Li, H.; Zhou, Z.-M.; Xu, Z.-H*. Nitrile-converting enzymes as a tool to improve biocatalysis in organic synthesis: Recent insights and promises. Critical Reviews in Biotechnology, 2017, 37 (1): 69-81. [20]Gong J-S, Dong T-T, Gu B-C, Li H, Dou W-F, Lu Z-M, Zhou Z-M, Shi J-S, Xu Z-H. Semirational engineering accelerates the laboratory evolution of nitrilase catalytic efficiency for nicotinic acid biosynthesis [J]. ChemCatChem 2017, 9 (17):3395-3401. [21]Gong J-S, Liu X-M, Zhang M-J, Li H, Geng Y, Li H, Li J, Lu Z-M, Xu Z-H, Shi J-S. Purification and characterization of a high salt-tolerant alginate lyase from Cobetia sp. WG-007 [J]. Biotechnology and Applied Biochemistry 2017, 64 (4):519-524. [22]Gong, J.-S.; Li, H.; Lu, Z.-M.; Zhang, X.-J.; Zhang, Q.; Yu, J.-H.; Zhou, Z.-M.; Shi, J.-S.; Xu, Z.-H. Engineering of a fungal nitrilase for improving catalytic activity and reducing by-product formation in the absence of structural information[J]. Catalysis Science and Technology, 2016, 6: 4134-4141. [23]Gong, J.-S.; Wang, Y.; Zhang, D.-D.; Zhang, R.-X.; Su, C.; Li, H.; Zhang, X.-M.; Xu, Z.-H.; Shi, J.-S. Biochemical characterization of an extreme alkaline and surfactant-stable keratinase derived from a newly isolated actinomycete Streptomyces aureofaciens k13[J]. RSC Advances 2015, 5, 24691-24699. [24]Gong J-S, Wang Y, Zhang D-D, Li H, Zhang X-M, Zhang R-X, Lu Z-M, Xu Z-H, Shi J-S. A Surfactant-stable Bacillus pumilus K9 alpha-Keratinase and Its Potential Application in Detergent Industry [J]. Chemical Research in Chinese Universities, 2015, 31 (1):91-97. [25]Gong, J.-S.; Li, W.; Zhang, D.-D.; Xie, M.-F.; Yang, B.; Zhang, R.-X.; Li, H.; Lu, Z.-M.; Xu, Z.-H.; Shi, J.-S. Biochemical characterization of an arginine-specific alkaline trypsin from bacillus licheniformis [J]. International Journal of Molecular Sciences 2015, 16, 30061-30074.
Selected Patents [1] A recombinant strain expressing phospholipase D and use thereof. US11377674B2, 2022-07-05 [2] An improved industrial keratinase via genetic engineering and use thereof. US11041160B2, 2021-06-22 [3] Strain of Enterobacter for degrading hyaluronic acid and application thereof. US11807847B2, 2023-11-07 [4] Directed modification of glucosamine synthase mutant and application thereof. US10233439 B2, 2019-03-19 [5] A recombinant nitrilase mutant via site-directed mutagenesis, ZL 201310069190.8 [6] A fungal nitrilase and its encoding sequence, ZL 201210089804.4 [7] Biotransformation of 3-cyanopyridine into nicotinic acid with Gibberella intermedia CA3-1, ZL 201110438755.6 [8]A strain of Pseudomonas putida and the conversion method for production of nicotinic acid or isonicotinic acid, ZL 201110116234.9 [9]The gene sequence of a nitrilase and its application, ZL 201210433096.1 [10] Screening of high glycine-producing strains and its application in nitrile biocatalysis, ZL 201010567150.2 [11]Immobilization of Gibberella intermedia with composite materials for producing nicotinic acid, ZL 20131063189.4 [12]A method for immobilization of Gibberella intermedia and biological preparation of nicotinic acid, CN 201210538372.0
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