Basic Information

Title: Professor

Office: 309, School of Life Sciences and Health Engineering, Jiangnan University

Email: lihui@jiangnan.edu.cn

Main Experiences

2002.9-2006.6 Nanjing Tech University, Pharmaceutical Engineering, Bachelor

2006.9-2011.6 Nanjing Tech University, Fermentation Engineering, PhD

2011.7-2021.7 Jiangnan University, School of Pharmaceutical Sciences, Lecturer, Associate Professor, Deputy Dean

2018.12-2019.12 Rensselaer Polytechnic Institute, USA, Visiting Scholar

2021.7-2022.1 Jiangnan University, School of Pharmaceutical Sciences, Professor, Deputy Dean

2022.1-present Jiangnan University, School of Life Sciences and Health Engineering, Professor, Deputy Dean

Research Areas

Biological synthesis and industrial application of important pharmaceutical intermediates, using synthetic biology, metabolic engineering, enzyme engineering, and other technical means, to analyze the biosynthesis mechanism of steroid hormone drugs, antibiotics, and vitamins, regulate and optimize metabolic pathways and fermentation processes, build efficient synthetic microbial cell factories, and realize their green manufacturing. In the past 5 years, she has presided over 6 projects including the National Natural Science Foundation projects, the National Key Research and Development Program of China, and enterprise horizontal cooperation projects; She has published more than 20 scientific research papers in authoritative journals in the domestic and overseas, authorized 8 national invention patents, and won 4 scientific research awards.

Awards and Honors

Second Prize of Scientific Research and Technological Inventions in Universities (2019)

First Prize of Science and Technology Progress Award of National Business Federation (2018)

Tianjin Science and Technology Progress Grand Prize (2020)

Jiangsu Province Universities “Qinglan Project” Outstanding Young Backbone Teacher Training object (2021)

Research Projects

National Natural Science Foundation of China, 2021-2024, presided

National Key Research and Development Program of China, 2021-2024, presided

Publications

[1] Liu W,Li H*, Guo DX, Ni Y, Zhang XM, Shi JS, Koffas MAG*, Xu ZH. Engineering of redox partners and cofactor NADPH supply of CYP68JX for efficient steroid two-step ordered selective hydroxylation activity,The Journal of Steroid Biochemistry and Molecular Biology, 2024, 238 106452-106452.

[2] He P,Li H*, Sun J, Zhang XM, Gong JS, Shi JS, Xu ZH. Identification of a fungal cytochrome P450 with steroid two-step ordered selective hydroxylation characteristics inColletotrichum lini,The Journal of Steroid Biochemistry and Molecular Biology, 2022, 220: 106096-106110.

[3]Li H, Shi WL, Li C, Zhang XM, Gong JS, Shi JS, Koffas MAG*, Xu ZH*. Impact of ethylene glycol on DHEA dihydroxylation inColletotrichum lini: Increasing the expression of cytochrome P450 and 6-phosphogluconate dehydrogenase and enhancing the generation of NADPH,Biochemical Engineering Journal, 2021, 166:107860-107867.

[4]Li H, Wu Y, Liu W, Zhang XM, Gong JS, Shi JS, Xu ZH*. iTRAQ-based quantitative proteomic analysis ofColletotrichum linireveals ethanol induced mechanism for enhancing dihydroxylation efficiency of DHEA,Journal of Proteomics, 2020, 224: 103851-103859.

[5] Zhou L,Li H*, Xu YN, Liu W, Zhang XM, Gong JS, Xu ZH, Shi JS*. Effects of a nonionic surfactant TX-40 on 9α-hydroxyandrost-4-ene-3,17-dione biosynthesis and physiological properties of Mycobacterium sp. LY-1. Process Biochemistry, 2019, 87: 89-94.

[6]Li H, Wang XD, Zhou LF, Ma Y, Yuan WJ, Zhang XM, Shi JS, Xu ZH. Enhancing expression of 3-ketosteroid-9α-hydroxylase oxygenase, an enzyme with broad substrate range and high hydroxylation ability, in Mycobacterium sp. LY-1. Applied Biochemistry and Biotechnology. 2019, 187(4):1238-1254.

Granted patents

[1] A method for the efficient synthesis of 15α-hydroxy-progesterone byColletotrichum lini, Issued number: ZL 201410683239.3.

[2] A method for promoting the regeneration of reducing power and improving the conversion efficiency of microbial hydroxylated DHEA, Issued number: ZL 201610082026.4.

[3] Cloning and Analysis of a 7α, 15α-dihydroxy DHEA P450 enzyme gene, Issued number: ZL 201310570323. X.

[4] A strain ofBacillus Nattowith high vitamin K2 (MK-7) production and its application, Issued number: ZL 201810392873. X.